follicle dermal papilla cell growth medium hfdpc  (PromoCell)

Journal: International Journal of Molecular Sciences

Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells

doi: 10.3390/ijms26052240

Figure Lengend Snippet: Veratric acid (VA) induces proliferation and growth signaling factors in HFDPCs. ( A ) Cell viability measured by MTT after HFDPCs were cultured with VA or minoxidil for 72 h. ( B ) Under the same condition for 48 h, cell proliferation was confirmed using Ki67 staining. ( C ) CCND1 and LEF1 mRNAs in HFDPCs treated with VA or minoxidil. ( D ) Western blot images of PCNA and β-catenin. All values were measured in triplicate (n = 3). Data are presented as mean ± SD. *, p < 0.05; **, p < 0.01.

Article Snippet: The human follicular dermal papilla cells (HFDPCs) were purchased from Promocell (Heidelberg, Germany) and cultured at 37 °C in a 5% CO 2 incubator using a follicle dermal papilla cell growth medium mixed with a growth medium supplement mix (Promocell, Heidelberg, Germany).

Techniques: Cell Culture, Staining, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells

doi: 10.3390/ijms26052240

Figure Lengend Snippet: Veratric acid increases hair inductivity-related factors. ( A ) Alkaline phosphatase staining of HFDPCs cultured with VA or minoxidil and graph of values measured using ImageJ. Scale bar = 1 μm. ( B ) Degree of aggregation of HFDPCs. Scale bar = 1000 μm. ( C ) Expression level of TGFB2 mRNA in HFDPCs. All values were measured in triplicate (n = 3). Data are presented as mean ± SD. *, p < 0.05; **, p < 0.01.

Article Snippet: The human follicular dermal papilla cells (HFDPCs) were purchased from Promocell (Heidelberg, Germany) and cultured at 37 °C in a 5% CO 2 incubator using a follicle dermal papilla cell growth medium mixed with a growth medium supplement mix (Promocell, Heidelberg, Germany).

Techniques: Staining, Cell Culture, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells

doi: 10.3390/ijms26052240

Figure Lengend Snippet: Veratric acid decreases apoptosis of HFDPCs. ( A ) Expression levels of BCL2 and BAX mRNAs in HFDPCs cultured with VA or minoxidil. *, p < 0.05. ( B ) Flow cytometry analysis of apoptosis of HFDPCs by Annexin V FITC and PI staining. *, p < 0.001. All values were measured in triplicate (n = 3). Data are presented as mean ± SD.

Article Snippet: The human follicular dermal papilla cells (HFDPCs) were purchased from Promocell (Heidelberg, Germany) and cultured at 37 °C in a 5% CO 2 incubator using a follicle dermal papilla cell growth medium mixed with a growth medium supplement mix (Promocell, Heidelberg, Germany).

Techniques: Expressing, Cell Culture, Flow Cytometry, Staining

Journal: International Journal of Molecular Sciences

Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells

doi: 10.3390/ijms26052240

Figure Lengend Snippet: Veratric acid reduces senescence in replicative senescent HFDPCs. ( A ) Flow cytometry analysis of β-gal activity in replicative senescent HFDPCs cultured with VA or EGCG. #, p -value < 0.01 vs. the young group; *, p -value < 0.01; **, p -value < 0.01 vs. the replicative senescence-induced untreated group (control group). ( B ) Expression levels of IL6 and TGFB1 mRNAs in replicative senescent HFDPCs. *, p -value < 0.05; **, p -value < 0.01. All values were measured in triplicate (n = 3). Data are presented as mean ± SD.

Article Snippet: The human follicular dermal papilla cells (HFDPCs) were purchased from Promocell (Heidelberg, Germany) and cultured at 37 °C in a 5% CO 2 incubator using a follicle dermal papilla cell growth medium mixed with a growth medium supplement mix (Promocell, Heidelberg, Germany).

Techniques: Flow Cytometry, Activity Assay, Cell Culture, Control, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells

doi: 10.3390/ijms26052240

Figure Lengend Snippet: Veratric acid alleviates senescence induced by oxidative stress on HFDPCs. ( A ) Flow cytometry analysis of β-gal activity. After pretreatment with 300 μM H 2 O 2 for 2 h, cells were incubated with VA for 72 h. β-gal activity was measured by FACS. ( B ) Expression levels of P21 and TGFB1 mRNAs in HFDPCs. All values were measured in triplicate (n = 3). Data are presented as mean ± SD. #, p -value < 0.05 vs. H 2 O 2 -untreated group; *, p -value < 0.05 vs. H 2 O 2 -treated group.

Article Snippet: The human follicular dermal papilla cells (HFDPCs) were purchased from Promocell (Heidelberg, Germany) and cultured at 37 °C in a 5% CO 2 incubator using a follicle dermal papilla cell growth medium mixed with a growth medium supplement mix (Promocell, Heidelberg, Germany).

Techniques: Flow Cytometry, Activity Assay, Incubation, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Anti-Hair Loss Effect of Veratric Acid on Dermal Papilla Cells

doi: 10.3390/ijms26052240

Figure Lengend Snippet: Hair growth mechanism of veratric acid in HFDPCs.

Article Snippet: The human follicular dermal papilla cells (HFDPCs) were purchased from Promocell (Heidelberg, Germany) and cultured at 37 °C in a 5% CO 2 incubator using a follicle dermal papilla cell growth medium mixed with a growth medium supplement mix (Promocell, Heidelberg, Germany).

Techniques: